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January 2015

Volume 1 Issue 1 


Focusing on The Main Morphological and Physiological Characteristics of the Food-Borne Pathogen Listeria monocytogenes

Domenico Meloni*

The genus Listeria spp includes fifteen species, i.e. L.monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L.seeligeri, L. grayi, L. marthii, L. rocourtiae, L. fleichmannii,L. weihenstephanensis, L. floridensis, L. aquatic, L. cornellensis, L. riparia and L. grandensis. Members of the genus Listeria are small Gram-positive rods sometimes arranged in short chains, ranging between 0.5 and 4 μm in diameter and between 0.5 and 2 μm in length.                                                                                 

                                                                                                                                          Download PDF Full text     __________________________________________________________________________________________________________

Research Article

Flunixin Meglumine Transdermal Pour-on Solution as Adjunct Therapy in The Treatment of Bovine Respiratory Disease in Calves Less Than 8 Weeks of Age

Julien Thiry* and Philippe Brianceau

The safety and efficacy of a new flunixin transdermal pour-on solution for cattle, used as an adjunct therapy in the treatment of bovine respiratory disease (BRD) in juvenile calves less than 8 weeks of age, was evaluated. A total of 49 calves of less than 8 weeks of age, showing severe signs of respiratory disease, were randomly assigned to treatment with either the test product, flunixin (Finadyne® Transdermal; MSD Animal Health) administered topically once along the dorsal midline, or a positive control product, carprofen (Rimadyl®; Zoetis) administered by subcutaneous injection once, on day 0. All animals received a long acting injection of cefquinome (Cobactan® LA 7.5%; MSD Animal Health) on day 0 and day 2.                                           

                                                                                                                                              Download PDF Full text__________________________________________________________________________________________________________

Research Article

Residue of Fipronil, S-Methoprene, and Amitraz in Dog Blood and in Gloves from Topical Certifect® Application: Toxicity and Safety Considerations 

Haley Nichols, Ramesh C. Gupta*, Robin B. Doss, Stephanie D. Bland, Terry D. Canerdy and James Zieren 

Currently, the use of ectoparasiticides on dogs to control ticks and fleas is inevitable, and the safety data for dogs, dog owners, and veterinary personnel are scarce. This investigation was therefore undertaken with two objectives: (1) to determine the residue of fipronil, s-methoprene, and amitraz in dog blood, and (2) to determine the transferable residues of these insecticides to gloves worn while petting experimental household dogs after the topical application of Certifect®. Certifect®(4.28 mL) contains 9.8% fipronil, 8.8% s-methoprene, and 22.1% amitraz.                     

                                                                                                                                       Download PDF Full text    __________________________________________________________________________________________________________

Research Article

Bronchoalveolar Lavage and Respiratory System Scoring of Normal Holstein Calves and Calves with Respiratory Disease 

Simon F. Peek*, Nick Keuler, Faye Hartmann, Alfonso Lago, Kenneth V. Nordlund, Sheila M. McGuirk 

Bronchoalveolar lavages (BALs) were performed on Holstein calves with (n=63) and without (n=63) clinical signs of respiratory disease on commercial dairy herds (n=24) in Wisconsin. Evaluation of respiratory disease was performed on farm for each calf using a scoring system that attributed cumulative number values to clinical parameters that are easily assessable by producers or veterinarians (fever, nasal and ocular discharge, presence or absence of a spontaneous or inducible cough).                                                                              

                                                                                                                                         Download PDF Full text    __________________________________________________________________________________________________________
Research Article

Epidemiology of Swine Hepatitis E Virus in Guangxi, China and Construction of Eukaryotic Expression Plasmid with ORF2 Gene for possible Vaccine

Q Su1#, B Li#, W Zhao*, Y. Qin, J Liang, B Lu, A Xiao, Q Duan, Y He, D Jiang, W Huang

Hepatitis E (HE) is a zoonotic disease that can be transmitted through food or water contaminated from swine with HE virus (HEV) as an important natural reservoir host. This paper presents emperical data of epidemiological testing conducted at 14 cities in Guangxi, China, where random samples of 1387 serum samples were collected from the swine population. ELISA test for total antibodies of HEV showed that 1107 were positive in swine serum samples, with an average positive rate of 79.81%. In order to develop swine hepatitis E DNA vaccine, specific primers of immunogenic specific ORF2 gene targets for LB1, LB2, LB3 were designed and amplified using PCR assays. DNA amplicons were then directly cloned into T-A plasmid and expressed using pEASY-M1 eukaryotic expression vector system.                                          

                                                                                                                                              Download PDF Full text__________________________________________________________________________________________________________

Review Article

Animal Welfare Considerations: Should Context Matter?

F. Ohl* and R.J. Putman

Much consideration of animal welfare issues, as well as the bulk of welfare legislation, is closely related to context, with clear distinction between defined responsibilities towards farm-, laboratory-, companion- and wild animals. It has however been recognised that such distinction leads to inconsistencies, in that it might lead to different welfare management practices for the same rat (for example) as a pet, laboratory animal or pest.                                            

                                                                                                                                              Download PDF Full text



Influence of 2,3,7,8-Tetrachlorodibenzo-P-Dioxin on Bovine Herpesvirus 1 Replication  

Filomena Fiorito*, Ugo Pagnini, Luisa De Martino and Giuseppe Iovane  

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most potent member of the dioxin group of chemicals, herein called dioxin, is a toxic and persistent environmental contaminant. Humans and animals are generally exposed to dioxin which is incorporated in food, mainly in products of animal origin rich in fat, but also in drinking water, soil, dust, smoke and air. TCDD exerts most of its biochemical effects by binding to the Aryl hydrocarbon Receptor (AhR). AhR forms a complex with the AhR nuclear translocator (ARNT) protein by interactions with dioxin responsive elements and activates the expression of a battery of genes that catalyzes the metabolism and conjugation of xenobiotics.

                                                                                                                                    Download PDF Full text ______________________________________________________________________________________________________


Polymorphic variants of the P2X7 receptor in the domestic dog

Ronald Sluyter* and Leanne Stokes

Due to distinctive features including genome architecture, population diversity, breed structure and breed-specific disorders, the domestic dog (Canis familiaris) is becoming an important animal to study the genetics of morphology, behaviour and disease susceptibility in mammals. Further, with around 360 of the some 450 diseases in dogs similar to that of human diseases, dogs provide a valuable model to identify the genetic causes of many diseases in humans.                                                                                

                                                                                                                                           Download PDF Full text 

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